2016
nonionic polyacrylamide gradient gel electrophoresis in united states
1056 BIOTECHNOLOGY- - US Pharmacopeia (USP)
Typically, analytical electrophoresis of for control of purity, and for quantitative determinations.proteins is carried out in polyacrylamide gels under condi- tions that ensure dissociation of the proteins into their indi- Purposevidual polypeptide subunits and that minimize aggregation.
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Methods of Low-Density Lipoprotein-Cholesterol Measurement
Abstract. Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size
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High density lipoproteins: Measurement techniques and
The gels do not contain any G-250. This system, based on the blue native polyacrylamide gel electrophoresis (BN PAGE) technique developed by Schägger and von Jagow, overcomes the limitations of traditional native gel electrophoresis by providing a near-neutral operating pH and detergent compatibility.
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Denaturing Gradient Gel Electrophoresis - an overview
J.P. Tamang, in Encyclopedia of Food Microbiology (Second Edition), 2014 Denaturing Gradient Gel Electrophoresis. Denaturing gradient gel electrophoresis (DGGE) is a method by which fragments of partial 16S rDNA-amplified fragments of identical length but different sequence can be resolved electrophoretically because of their different melting behavior in a gel system containing a gradient of
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Characterization of ZO-1, a protein component of the tight
ZO-1, originally identified by mAb techniques, is the first protein shown to be specifically associated with the tight junction. Here we describe and compare the physical characteristics of ZO-1 from mouse liver and the Madin-Darby canine kidney (MDCK) epithelial cell line.
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Gradient polyacrylamide gel electrophoresis for determination
We report the use of gradient polyacrylamide gel electrophoresis (PAGE) to determine the M(r) values of pharmaceutical heparins and LMW heparin derivatives. This approach offers certain advantages over the HPLC method. Gradient PAGE analysis was performed in parallel, on multiple samples, with the same standard curve. HPLC was performed serially.
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A rapid 3% polyacrylamide slab gel electrophoresis method for
The apparent molecular weights of the heat-resistant esterase of Y. enterocolitica and of the major heat-resistant esterase of Y. pseudotuberculosis, as determined by polyacrylamide gradient gel electrophoresis, were estimated to be 52 000 and 250 000, respectively.
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Nondenaturing electrophoresis of lipoproteins in agarose and
Nondenaturing electrophoresis in agarose gels and polyacrylamide gradient gels are complementary analytical methods for classification of lipoproteins and determining distribution profiles of the major classes. In addition, gradient gel electrophoresis (GGE) has a high resolving capability for subfractionating each class according to particle size.
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Isolation and recovery of acidic oligosaccharides from
By using multiple membrane layers a second separation dimension was obtained, resulting in increased oligosaccharide purity proportional to transfer depth. Preparative gradient polyacrylamide gel electrophoresis followed by semi-dry electro-transfer and recovery represents a novel method for the preparation of homogeneous acidic oligosaccharides.
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Non-Gradient Blue Native Polyacrylamide Gel Electrophoresis
(2-D) electrophoresis can be grouped under the term “protein electrophoresis” (Rabilloud 2010). Though some information is provided about these methods in the following chapters, this guide focuses on the one-dimensional separation of proteins in polyacrylamide gels, or polyacrylamide gel electrophoresis (PAGE). Fig. 1.2.
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